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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has become observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial role in endorsing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been claimed but thorough nucleotide sequence info is not really nevertheless offered.

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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide usually includes the epitope identified by the antibody.

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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best effects are...

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This guide outlines additional achievable optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...

Antibodies bound to the blocking peptide now not bind into more info the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody on your own, you can see which staining is precise.

Focus on Details Cyclin E belongs towards the highly conserved cyclin spouse and children, whose associates are characterized by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins exhibit unique expression and degradation styles which lead into the temporal coordination of every mitotic party. Cyclin E types a posh with and functions to be a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates for the G1-S phase boundary and is also degraded as cells development through S period.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...

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